Locked nucleic acid containing antisense oligonucleotides enhance inhibition of HIV-1 genome dimerization and inhibit virus replication.
Identifieur interne : 000324 ( Ncbi/Merge ); précédent : 000323; suivant : 000325Locked nucleic acid containing antisense oligonucleotides enhance inhibition of HIV-1 genome dimerization and inhibit virus replication.
Auteurs : Joacim Elmén [Suède] ; Hong-Yan Zhang ; Bartek Zuber ; Karl Ljungberg ; Britta Wahren ; Claes Wahlestedt ; Zicai LiangSource :
- FEBS letters [ 0014-5793 ] ; 2004.
Descripteurs français
- KwdFr :
- Activation enzymatique, Cellules Jurkat, Dimérisation, Génome viral, Humains, Lymphocytes T (), Lymphocytes T (métabolisme), Oligonucléotides antisens (), Oligonucléotides antisens (pharmacologie), Ribonuclease H (métabolisme), Réplication virale (), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (isolement et purification).
- MESH :
- génétique : VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- isolement et purification : VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- métabolisme : Lymphocytes T, Ribonuclease H.
- pharmacologie : Oligonucléotides antisens.
- Activation enzymatique, Cellules Jurkat, Dimérisation, Génome viral, Humains, Lymphocytes T, Oligonucléotides antisens, Réplication virale, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
English descriptors
- KwdEn :
- Dimerization, Enzyme Activation, Genome, Viral, HIV-1 (drug effects), HIV-1 (genetics), HIV-1 (isolation & purification), Humans, Jurkat Cells, Oligonucleotides, Antisense (chemistry), Oligonucleotides, Antisense (pharmacology), Ribonuclease H (metabolism), T-Lymphocytes (drug effects), T-Lymphocytes (metabolism), Virus Replication (drug effects).
- MESH :
- chemical , chemistry : Oligonucleotides, Antisense.
- drug effects : HIV-1, T-Lymphocytes, Virus Replication.
- genetics : HIV-1.
- isolation & purification : HIV-1.
- chemical , metabolism : Ribonuclease H, T-Lymphocytes.
- chemical , pharmacology : Oligonucleotides, Antisense.
- Dimerization, Enzyme Activation, Genome, Viral, Humans, Jurkat Cells.
Abstract
We have evaluated antisense design and efficacy of locked nucleic acid (LNA) and DNA oligonucleotide (ON) mix-mers targeting the conserved HIV-1 dimerization initiation site (DIS). LNA is a high affinity nucleotide analog, nuclease resistant and elicits minimal toxicity. We show that inclusion of LNA bases in antisense ONs augments the interference of HIV-1 genome dimerization. We also demonstrate the concomitant RNase H activation by six consecutive DNA bases in an LNA/DNA mix-mer. We show ON uptake via receptor-mediated transfection of a human T-cell line in which the mix-mers subsequently inhibit replication of a clinical HIV-1 isolate. Thus, the technique of LNA/DNA mix-mer antisense ONs targeting the conserved HIV-1 DIS region may provide a strategy to prevent HIV-1 assembly in the clinic.
DOI: 10.1016/j.febslet.2004.11.015
PubMed: 15589834
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pubmed:15589834Le document en format XML
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first="Bartek" last="Zuber">Bartek Zuber</name></author><author><name sortKey="Ljungberg, Karl" sort="Ljungberg, Karl" uniqKey="Ljungberg K" first="Karl" last="Ljungberg">Karl Ljungberg</name></author><author><name sortKey="Wahren, Britta" sort="Wahren, Britta" uniqKey="Wahren B" first="Britta" last="Wahren">Britta Wahren</name></author><author><name sortKey="Wahlestedt, Claes" sort="Wahlestedt, Claes" uniqKey="Wahlestedt C" first="Claes" last="Wahlestedt">Claes Wahlestedt</name></author><author><name sortKey="Liang, Zicai" sort="Liang, Zicai" uniqKey="Liang Z" first="Zicai" last="Liang">Zicai Liang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15589834</idno><idno type="pmid">15589834</idno><idno type="doi">10.1016/j.febslet.2004.11.015</idno><idno type="wicri:Area/PubMed/Corpus">002353</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" 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Karolinska Institutet, Berzeliusväg 35, 171 77 Stockholm</wicri:regionArea><wicri:noRegion>171 77 Stockholm</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Hong Yan" sort="Zhang, Hong Yan" uniqKey="Zhang H" first="Hong-Yan" last="Zhang">Hong-Yan Zhang</name></author><author><name sortKey="Zuber, Bartek" sort="Zuber, Bartek" uniqKey="Zuber B" first="Bartek" last="Zuber">Bartek Zuber</name></author><author><name sortKey="Ljungberg, Karl" sort="Ljungberg, Karl" uniqKey="Ljungberg K" first="Karl" last="Ljungberg">Karl Ljungberg</name></author><author><name sortKey="Wahren, Britta" sort="Wahren, Britta" uniqKey="Wahren B" first="Britta" last="Wahren">Britta Wahren</name></author><author><name sortKey="Wahlestedt, Claes" sort="Wahlestedt, Claes" uniqKey="Wahlestedt C" first="Claes" last="Wahlestedt">Claes Wahlestedt</name></author><author><name sortKey="Liang, Zicai" sort="Liang, Zicai" uniqKey="Liang Z" first="Zicai" last="Liang">Zicai 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()</term><term>Lymphocytes T (métabolisme)</term><term>Oligonucléotides antisens ()</term><term>Oligonucléotides antisens (pharmacologie)</term><term>Ribonuclease H (métabolisme)</term><term>Réplication virale ()</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) ()</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique)</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Oligonucleotides, Antisense</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>HIV-1</term><term>T-Lymphocytes</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV-1</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>HIV-1</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Ribonuclease H</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lymphocytes T</term><term>Ribonuclease H</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Oligonucléotides antisens</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Oligonucleotides, Antisense</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Dimerization</term><term>Enzyme Activation</term><term>Genome, Viral</term><term>Humans</term><term>Jurkat Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation 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LNA is a high affinity nucleotide analog, nuclease resistant and elicits minimal toxicity. We show that inclusion of LNA bases in antisense ONs augments the interference of HIV-1 genome dimerization. We also demonstrate the concomitant RNase H activation by six consecutive DNA bases in an LNA/DNA mix-mer. We show ON uptake via receptor-mediated transfection of a human T-cell line in which the mix-mers subsequently inhibit replication of a clinical HIV-1 isolate. Thus, the technique of LNA/DNA mix-mer antisense ONs targeting the conserved HIV-1 DIS region may provide a strategy to prevent HIV-1 assembly in the clinic.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15589834</PMID><DateCompleted><Year>2005</Year><Month>01</Month><Day>28</Day></DateCompleted><DateRevised><Year>2007</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0014-5793</ISSN><JournalIssue CitedMedium="Print"><Volume>578</Volume><Issue>3</Issue><PubDate><Year>2004</Year><Month>Dec</Month><Day>17</Day></PubDate></JournalIssue><Title>FEBS letters</Title><ISOAbbreviation>FEBS Lett.</ISOAbbreviation></Journal><ArticleTitle>Locked nucleic acid containing antisense oligonucleotides enhance inhibition of HIV-1 genome dimerization and inhibit virus replication.</ArticleTitle><Pagination><MedlinePgn>285-90</MedlinePgn></Pagination><Abstract><AbstractText>We have evaluated antisense design and efficacy of locked nucleic acid (LNA) and DNA oligonucleotide (ON) mix-mers targeting the conserved HIV-1 dimerization initiation site (DIS). LNA is a high affinity nucleotide analog, nuclease resistant and elicits minimal toxicity. We show that inclusion of LNA bases in antisense ONs augments the interference of HIV-1 genome dimerization. We also demonstrate the concomitant RNase H activation by six consecutive DNA bases in an LNA/DNA mix-mer. We show ON uptake via receptor-mediated transfection of a human T-cell line in which the mix-mers subsequently inhibit replication of a clinical HIV-1 isolate. Thus, the technique of LNA/DNA mix-mer antisense ONs targeting the conserved HIV-1 DIS region may provide a strategy to prevent HIV-1 assembly in the clinic.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Elmén</LastName><ForeName>Joacim</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Center for Genomics and Bioinformatics, Karolinska Institutet, Berzeliusväg 35, 171 77 Stockholm, Sweden. joacim.elmen@cgb.ki.se</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Hong-Yan</ForeName><Initials>HY</Initials></Author><Author ValidYN="Y"><LastName>Zuber</LastName><ForeName>Bartek</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Ljungberg</LastName><ForeName>Karl</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Wahren</LastName><ForeName>Britta</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Wahlestedt</LastName><ForeName>Claes</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Zicai</ForeName><Initials>Z</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>FEBS Lett</MedlineTA><NlmUniqueID>0155157</NlmUniqueID><ISSNLinking>0014-5793</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016376">Oligonucleotides, Antisense</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.26.4</RegistryNumber><NameOfSubstance UI="D016914">Ribonuclease H</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D019281" MajorTopicYN="N">Dimerization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004789" MajorTopicYN="N">Enzyme Activation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016679" MajorTopicYN="Y">Genome, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015497" MajorTopicYN="N">HIV-1</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019169" MajorTopicYN="N">Jurkat Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016376" MajorTopicYN="N">Oligonucleotides, Antisense</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016914" MajorTopicYN="N">Ribonuclease H</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013601" MajorTopicYN="N">T-Lymphocytes</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2004</Year><Month>09</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2004</Year><Month>11</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2004</Year><Month>11</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>12</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>1</Month><Day>29</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>12</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15589834</ArticleId><ArticleId IdType="pii">S0014-5793(04)01379-1</ArticleId><ArticleId IdType="doi">10.1016/j.febslet.2004.11.015</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country></list><tree><noCountry><name sortKey="Liang, Zicai" sort="Liang, Zicai" uniqKey="Liang Z" first="Zicai" last="Liang">Zicai Liang</name><name sortKey="Ljungberg, Karl" sort="Ljungberg, Karl" uniqKey="Ljungberg K" first="Karl" last="Ljungberg">Karl Ljungberg</name><name sortKey="Wahlestedt, Claes" sort="Wahlestedt, Claes" uniqKey="Wahlestedt C" first="Claes" last="Wahlestedt">Claes Wahlestedt</name><name sortKey="Wahren, Britta" sort="Wahren, Britta" uniqKey="Wahren B" first="Britta" last="Wahren">Britta Wahren</name><name sortKey="Zhang, Hong Yan" sort="Zhang, Hong Yan" uniqKey="Zhang H" first="Hong-Yan" last="Zhang">Hong-Yan Zhang</name><name sortKey="Zuber, Bartek" sort="Zuber, Bartek" uniqKey="Zuber B" first="Bartek" last="Zuber">Bartek Zuber</name></noCountry><country name="Suède"><noRegion><name sortKey="Elmen, Joacim" sort="Elmen, Joacim" uniqKey="Elmen J" first="Joacim" last="Elmén">Joacim Elmén</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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